Open AccessOmega-amidase pathway in the degradation of glutamine in Neurospora crassa
Author(s) -
J Calderón,
Enrique Morett,
Jaime Font de Mora
Publication year - 1985
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.161.2.807-809.1985
Subject(s) - aminooxyacetic acid , neurospora crassa , biology , glutamine , biochemistry , glutamate synthase , amidase , neurospora , transaminase , glutamine synthetase , glutamate dehydrogenase , glutaminase , enzyme , glutamate receptor , amino acid , mutant , gene , receptor
Evidence for the participation of the glutamine transaminase-omega-amidase pathway in the utilization of glutamine in Neurospora crassa was obtained. Its participation is indicated by the in vitro activities of glutamine transaminase and omega-amidase, the in vivo accumulation of alpha-ketoglutaramate when an inhibitor of transamidases is present, and the inhibition by aminooxyacetic acid and 6-diazo-5-oxo-L-norleucine of the ammonium excreted in the presence of glutamine by a mutant strain that lacks glutamate dehydrogenase and glutamate synthase.
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