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We inserted all but the extreme 5′ end of a DNA copy of the bacteriophage MS2 lysis gene downstream of alac -induced promoter on a multicopy plasmid. Upon induction, cells harboring this plasmid began to lyse, showing that phage coat protein is not required for the lytic process itself.

Bacteriophage MS2 Lysis Protein Does Not Require Coat Protein to Mediate Cell Lysis