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Mutant strains ofNeurospora crassa have been selected which grow on media containing vanadate, an inhibitor of the plasma membrane ATPase. The mutations all map to a single region (designatedvan ) on the left arm of linkage group VII. Thevan mutants are unable to take up vanadate from the medium and are also deficient in the uptake of phosphate via a derepressible, high-affinity phosphate transport system. In thevan mutants, theKm for phosphate transport is elevated as much as 35-fold, indicating that thevan locus may code for a structural component of the high-affinity phosphate transport system.

Vanadate-Resistant Mutants of Neurospora crassa Are Deficient in a High-Affinity Phosphate Transport System