Open AccessCloning of immunity and structural genes for colicin V
Author(s) -
Kevin K. Frick,
Robert L. Quackenbush,
J Konisky
Publication year - 1981
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.148.2.498-507.1981
Subject(s) - colicin , biology , plasmid , transposable element , pbr322 , gene , cloning (programming) , mutant , genetics , immunity , cloning vector , structural gene , molecular cloning , escherichia coli , microbiology and biotechnology , peptide sequence , immune system , programming language , computer science
The colicin V immunity and structural genes of plasmid pColV-B188 were cloned into the vectors pMB9, pBR322, and pMK16. Both genes are closely linked and can be isolated on a 900-base-pair deoxyribonucleic acid fragment. Insertion of the transposon Tn5 into this cloned sequence led to the construction of a mutant plasmid which conferred colicin V immunity, but not the ability to produce this colicin. Analysis of the products determined by these cloned genes in cells has led to the conclusion that the polypeptide involved in immunity has a molecular weight of about 6,500, whereas the colicin has a molecular weight of approximately 4,000.
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