Open AccessIdentification of the tetracycline resistance promoter and repressor in transposon Tn10
Author(s) -
Lewis V. Wray,
Richard A. Jorgensen,
W S Reznikoff
Publication year - 1981
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.147.2.297-304.1981
Subject(s) - tn10 , biology , transposable element , tetracycline , repressor , plasmid , genetics , dna transposable elements , gene , base pair , transposition (logic) , restriction map , microbiology and biotechnology , gene expression , mutant , antibiotics , linguistics , philosophy
The structural and regulatory functions encoding tetracycline resistance in transposon Tn10 lie within a 2,700-base pair region. Using recombinant plasmids with different deoxyribonucleic acid sequences adjacent to a HincII site in this region, we located the promoter controlling the expression of tetracycline resistance. These various sequences conferred altered levels of tetracycline resistance. Plasmids containing deletions of a 695-base pair HincII fragment were constitutive and showed the loss of a 23,000-dalton tetracycline-inducible polypeptide, thus identifying the repressor and the location of its gene.
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