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The transforming activity ofBacillus subtilis deoxyribonucleic acid (DNA) that had been sheared and purified with respect to size by sucrose gradient sedimentation is given as a function of the DNA molecular weight. It is shown (i) that fragments of median molecular weight 1.2 × 106 have finite activity (10−4 ), (ii) that the shape of the activity-versus-molecular weight function is qualitatively similar to that observed previously forDiplococcus pneumoniae , and (iii) that this shape precludes interpretation in terms of critical size models.

Activity of Deoxyribonucleic Acid Fragments of Defined Size in Bacillus subtilis Transformation