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A neomycin-resistant mutant of Escherichia coli K-12 unable to grow on Krebs cycle intermediates has been isolated. The mutant retained its respiratory capacity, but lacked membrane Mg(2+)-Ca(2+)-stimulated adenosine triphosphatase activity (EC 3.6.1.3).

Use of Neomycin in the Isolation of Mutants Blocked in Energy Conservation in Escherichia coli