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A technique allowing chain formation byEscherichia coli for the analysis of the segregation of certain cellular components by autoradiographic methods has been developed. Deoxyribonucleic acid labeled with3 H-thymidine does not appear to segregate preferentially with one or another of the bacteria. Membrane lipids labeled with either3 H-oleate or3 H-glycerol are evenly distributed among all progeny cells. A similar pattern of distribution of cell wall material is observed when3 H-diaminopimelate is used as the marker.

journal of bacteriology

On the Process of Cellular Division in <i>Escherichia coli</i> VI. Use of a Methocel-Autoradiographic Method for the Study of Cellular Division in <i>Escherichia coli</i>

On the Process of Cellular Division in Escherichia coli VI. Use of a Methocel-Autoradiographic Method for the Study of Cellular Division in Escherichia coli