English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Lateral gene transfer (LGT) is essential for generating between-strain genomic recombinants ofChlamydia trachomatis to facilitate the organism's evolution. Because there is no reliable laboratory-based gene transfer system forC. trachomatis ,in vitro generation of recombinants from antibiotic-resistant strains is being used to study LGT. However, selection pressures imposed onin vitro recombinants likely affect statistical properties of recombination relative to naturally occurring clinical recombinants, including prevalence at particular loci. We examined multiple loci for 16in vitro -derived recombinants of ofloxacin- and rifampin-resistant L1 and D strains, respectively, grown with both antibiotics, and compared these with the same sequenced loci among 11 clinical recombinants. Breakpoints and recombination frequency were examined using phylogenetics, bioinformatics, and statistics.In vitro and clinical isolates clustered perfectly into two groups, without misclassification, using Ward's minimum variance based on breakpoint data. As expected,gyrA (confers ofloxacin resistance) andrpoB (confers rifampin resistance) had significantly more breakpoints amongin vitro recombinants than among clinical recombinants (P &lt; 0.0001 andP = 0.02, respectively, using the Wilcoxon rank sum test). Unexpectedly,trpA also had significantly more breakpoints forin vitro recombinants (P &lt; 0.0001). There was also significant selection at other loci. The strongest bias was forompA in strain D (P = 3.3 × 10−8 ). Our results indicate that thein vitro model differs statistically from natural recombination events. Additional genomic studies are needed to determine the factors responsible for the observed selection biases at unexpected loci and whether these are important for LGT to inform approaches for genetically manipulatingC. trachomatis .

journal of bacteriology

In Vitro Recombinants of Antibiotic-Resistant Chlamydia trachomatis Strains Have Statistically More Breakpoints than Clinical Recombinants for the Same Sequenced Loci and Exhibit Selection at Unexpected Loci