English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

As a result of a frameshift mutation, thehsdS locus of the NgoAV type IC restriction and modification (RM) system comprises two genes,hsdS NgoAV1 andhsdS NgoAV2 . The specificity subunit, HsdSNgoAV , the product of thehsdS NgoAV1 gene, is a naturally truncated form of an archetypal specificity subunit (208 N-terminal amino acids instead of 410). The presence of a homonucleotide tract of seven guanines (poly[G]) at the 3′ end of thehsdS NgoAV1 gene makes the NgoAV system a strong candidate for phase variation, i.e., stochastic addition or reduction in the guanine number. We have constructed mutants with 6 guanines instead of 7 and demonstrated that the deletion of a single nucleotide within the 3′ end of thehsdS NgoAV1 gene restored the fusion between thehsdS NgoAV1 andhsdS NgoAV2 genes. We have demonstrated that such a contraction of the homonucleotide tract may occurin vivo : in aNeisseria gonorrhoeae population, a minor subpopulation of cells appeared to have only 6 guanines at the 3′ end of thehsdS NgoAV1 gene.Escherichia coli cells carrying the fused gene and expressing the NgoAVΔ RM system were able to restrict λ phage at a level comparable to that for the wild-type NgoAV system. NgoAV recognizes the quasipalindromic interrupted sequence 5′-GCA(N8 )TGC-3′ and methylates both strands. NgoAVΔ recognizes DNA sequences 5′-GCA(N7 )GTCA-3′ and 5′-GCA(N7 )CTCA-3′, although the latter sequence is methylated only on the complementary strand within the 5′-CTCA-3′ region of the second recognition target sequence.

journal of bacteriology

Deletion of One Nucleotide within the Homonucleotide Tract Present in the <i>hsdS</i> Gene Alters the DNA Sequence Specificity of Type I Restriction-Modification System NgoAV

Deletion of One Nucleotide within the Homonucleotide Tract Present in the hsdS Gene Alters the DNA Sequence Specificity of Type I Restriction-Modification System NgoAV