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Escherichia coli FNR is an O(2)-sensing transcription factor. In vitro studies indicate that anaerobic iron-sulfur cluster acquisition promotes FNR dimerization. Here, two-hybrid assays show that iron-sulfur cluster-dependent FNR dimers are formed in vivo in response to lower O(2) availability, consistent with the current model of FNR activation.

In Vivo Demonstration of FNR Dimers in Response to Lower O 2 Availability

In Vivo Demonstration of FNR Dimers in Response to Lower O ₂ Availability