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We report the 4.0-Mb genome sequence ofBacillus isronensis strain B3W22 isolated from air collected at an altitude ranging from 27 to 30 km above the city of Hyderabad, in India. This genome sequence will contribute to the objective of determining the microbial diversity of the upper atmosphere.

Draft Genome Sequence of Bacillus isronensis Strain B3W22, Isolated from the Upper Atmosphere