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The role of the N-terminal region of the transcription factor FNR, which immediately precedes the first ligand (Cys20) of the [4Fe-4S] cluster, was investigated. We found that truncation mutants that removed residues 2 to 16 and 2 to 17 had wild-type levels of FNR protein but surprisingly altered O(2) regulation.

Dissecting the Role of the N-Terminal Region of the Escherichia coli Global Transcription Factor FNR