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In the present study, we report the identification of a putative enoyl-coenzyme A (CoA) hydratase/isomerase that is required for synthesis of the biofilm dispersion autoinducercis -2-decenoic acid in the human pathogenPseudomonas aeruginosa . The protein is encoded by PA14_54640 (PA0745), nameddspI ford isp ersioni nducer. The gene sequence for this protein shows significant homology to RpfF inXanthomonas campestris . Inactivation ofdspI was shown to abolish biofilm dispersion autoinduction in continuous cultures ofP. aeruginosa and resulted in biofilms that were significantly greater in thickness and biomass than those of the parental wild-type strain. Dispersion was shown to be inducible indspI mutants by the exogenous addition of syntheticcis -2-decenoic acid or by complementation of ΔdspI in trans under the control of an arabinose-inducible promoter. Mutation ofdspI was also shown to abolishcis -2-decenoic acid production, as revealed by gas chromatography-mass spectrometry (GC-MS) analysis of cell-free spent culture medium. The transcript abundance ofdspI correlated with cell density, as determined by quantitative reverse transcriptase (RT) PCR. This regulation is consistent with the characterization ofcis -2-decenoic acid as a cell-to-cell communication molecule that regulates biofilm dispersion in a cell density-dependent manner.

journal of bacteriology

The Putative Enoyl-Coenzyme A Hydratase DspI Is Required for Production of the Pseudomonas aeruginosa Biofilm Dispersion Autoinducer <i>cis</i> -2-Decenoic Acid

The Putative Enoyl-Coenzyme A Hydratase DspI Is Required for Production of the Pseudomonas aeruginosa Biofilm Dispersion Autoinducer cis -2-Decenoic Acid