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Expression of pyrimidinede novo biosynthesis is downregulated by an exogenous uracil in many bacteria. In this study, we show that a putative binding motif sequence of PyrR is required for uracil-mediated repression ofpyrR-lacZ translational fusion. However, the uracil response was still observed in the strain with thepyrR gene deleted, implying the existence of a uracil response factor other than PyrR which also acts through the PyrR binding loop region. Deletion ofrho , encoding the transcription termination factor Rho, resulted in an increase in the expression ofpyrR-lacZ . Moreover, the strain with a double deletion ofpyrR andrho showed elimination of the uracil-responsive downregulation of thepyrR-lacZ . Therefore, expression of the pyrimidine biosynthetic gene cluster inCorynebacterium glutamicum is controlled by two different mechanisms mediated by PyrR and Rho.IMPORTANCE Thepyr genes ofC. glutamicum are downregulated in the presence of uracil in culture medium. The mRNA binding regulator PyrR represses the expression ofpyr genes, as reported previously. However, the uracil response was still observed in thepyrR deletion strain. Deletion ofrho in addition topyrR deletion results in the elimination of the uracil response. Therefore, we identified the factors that are involved in the uracil response. Involvement of Rho in the regulation of pyrimidinede novo biosynthesis genes has not been reported.

Regulation of the Expression of De Novo Pyrimidine Biosynthesis Genes in Corynebacterium glutamicum