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Guinea pigs were immunized by intramuscular injection of arthrospores from the M11 strain ofCoccidioides immitis , and the peritoneal exudate cells were harvested 4 to 6 weeks later. After incubation with various concentrations of coccidioidin in tissue culture, the area of all migration was measured. Results of this study indicate that at a critical level, a variance of 0.1 μg of antigen per ml, determined the difference between approximately 88% migration and 5% migration as compared with control cells incubated in the absence of antigen. The concentration of antigen (ASU-9 stock coccidioidin concentrate) required to produce essentially complete inhibition of migration was determined to be 12.5 μg/ml.

Variation in the In Vitro Migration of Sensitized Guinea Pig Peritoneal Exudate Cells in the Presence of Coccidioidin