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ESAT-6 is a virulence determinant in Mycobacterium tuberculosis and a member of a conserved group of proteins in a variety of other bacteria. A targeted deletion of the homologous gene in Listeria was generated, and in contrast to that observed for mycobacteria, this locus was not required for Listeria virulence.

The Mycobacterium tuberculosis ESAT-6 Homologue in Listeria monocytogenes Is Dispensable for Growth In Vitro and In Vivo