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Little is known about fungal biofilms, which may cause infection and antibiotic resistance. In this study, biofilm formation by differentCandida species, particularlyCandida albicans andC .parapsilosis , was evaluated by using a clinically relevant model ofCandida biofilm on medical devices.Candida biofilms were allowed to form on silicone elastomer and were quantified by tetrazolium (XTT) and dry weight (DW) assays. Formed biofilm was visualized by using fluorescence microscopy and confocal scanning laser microscopy with Calcofluor White (Sigma Chemical Co., St. Louis, Mo.), concanavalin A-Alexafluor 488 (Molecular Probes, Eugene, Oreg.), and FUN-1 (Molecular Probes) dyes. Although minimal variations in biofilm production among invasiveC .albicans isolates were seen, significant differences between invasive and noninvasive isolates (P &lt; 0.001) were noted.C .albicans isolates produced more biofilm thanC .parapsilosis ,C .glabrata , andC .tropicalis isolates, as determined by DW assays (P was &lt;0.001 for all comparisons) and microscopy. Interestingly, noninvasive isolates demonstrated a higher level of XTT activity than invasive isolates. On microscopy,C .albicans biofilms had a morphology different from that of other species, consisting of a basal blastospore layer with a dense overlying matrix composed of exopolysaccharides and hyphae. In contrast,C .parapsilosis biofilms had less volume thanC .albicans biofilms and were comprised exclusively of clumped blastospores. Unlike planktonically grown cells,Candida biofilms rapidly (within 6 h) developed fluconazole resistance (MIC, &gt;128 μg/ml). Importantly, XTT and FUN-1 activity showed biofilm cells to be metabolically active. In conclusion, our data show thatC .albicans produces quantitatively larger and qualitatively more complex biofilms than other species, in particular,C .parapsilosis .

Comparison of Biofilms Formed byCandidaalbicansandCandidaparapsilosison Bioprosthetic Surfaces