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To determine the importance of the O75 O antigen and the K5 capsular antigen in resistance to phagocytosis and phagocytic killing, we used previously described O75− and K5− mutants from an O75+ K5+ wild-type uropathogenicEscherichia coli strain in phagocytosis assays with polymorphonuclear leukocytes (PMNs) and monocytes. At a 10-to-1 ratio of bacteria to phagocytes and in the presence of 10% serum, the parental strain GR-12 was resistant to both PMNs and monocytes over a 2-h incubation period. The O75− and K5− mutants were similar in sensitivity to killing by both PMNs and monocytes, decreasing in viability by 80% in the first hour. Yet, a significant difference in killing between the O75− and K5− mutants was observed in the first 15 min of incubation. The K5− mutant decreased in numbers by almost 60%, while the O75− mutant increased in numbers similarly to GR-12 in the first 15 min. The difference in killing was found not to be due to the rate of opsonization. To further determine the mechanism of resistance, a fluorescence assay was used to differentiate attached and internalized bacteria. The K5 capsule hindered the association of both the wild-type strain and the O75− mutant in the initial incubation time with PMNs. In conclusion, both the K5 capsule and O75 O antigen play crucial roles in resistance to phagocytosis over time.

Loss of Resistance to Ingestion and Phagocytic Killing by O−and K−Mutants of a UropathogenicEscherichia coliO75:K5 Strain

Loss of Resistance to Ingestion and Phagocytic Killing by O⁻and K⁻Mutants of a UropathogenicEscherichia coliO75:K5 Strain