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The DNA sequence of the O-antigen biosynthesis cluster (wbf ) of a recently emergent pathogen,Vibrio cholerae serogroup O139, has been determined. Here we report the sequence of the genes downstream of the O139wbfX gene and analysis of the genes flanking thewbf gene cluster in other serogroups. The gene downstream ofwbfX , designatedrjg (right junction gene), is predicted to be not required for O-antigen biosynthesis but appears to be a hot spot for DNA rearrangements. Several variants of therjg gene (three different insertions and a deletion) have been found in other serogroups. DNA dot blot analysis of 106V. cholerae strains showed the presence of the left and right junction genes,gmhD andrjg , respectively, in all strains. Further, these genes mapped to a single I-Ceu I fragment in all 21 strains analyzed by pulsed-field gel electrophoresis, indicating a close linkage. The insertion sequence element IS1358 , found in both O1 and O139wb * regions, is present in 61% of the strains tested; interestingly, where present, it is predominantly linked to thewb * region. These results indicated a cassette-like organization of thewb * region, with the conserved genes (gmhD andrjg ) flanking the divergent, serogroup-specificwb * genes and IS1358 . A similar organization of thewb * region in other serogroups raises the possibility of the emergence of new pathogens by homologous recombination via the junction genes.

infection and immunity

Cloning and Sequencing of the Genes Downstream of the <i>wbf</i> Gene Cluster of <i>Vibrio cholerae</i> Serogroup O139 and Analysis of the Junction Genes in Other Serogroups

Cloning and Sequencing of the Genes Downstream of the wbf Gene Cluster of Vibrio cholerae Serogroup O139 and Analysis of the Junction Genes in Other Serogroups