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Adherence to the endothelial cell lining of the vasculature is probably a critical step in the egress ofCandida albicans from the intravascular compartment. To identify potential adhesins that mediate the attachment of this organism to endothelial cells, a genomic library fromC. albicans was used to transform a nonadherent strain ofSaccharomyces cerevisiae . The population of transformed yeasts was enriched for highly adherent clones by repeated passages over endothelial cells. One clone which exhibited a fivefold increase in endothelial cell adherence, compared withS. cerevisiae transformed with vector alone, was identified. This organism also flocculated. The candidal DNA fragment within this adherent/flocculent organism was found to contain a single 1.8-kb open reading frame, which was designatedCAD1 . It was found to be identical toAAF1 . The predicted protein encoded byCAD1/AAF1 contained features suggestive of a regulatory factor. Consistent with this finding, immunoelectron microscopy revealed that CAD1/AAF1 localized to the cytoplasm and nucleus but not the cell wall or plasma membrane of the transformed yeasts. Because yeasts transformed withCAD1/AAF1 both flocculated and exhibited increased endothelial cell adherence, the relationship between adherence and flocculation was examined.S. cerevisiae expressing either of two flocculation phenotypes, Flo1 or NewFlo, adhered to endothelial cells as avidly as did yeasts expressingCAD1/AAF1 . Inhibition studies revealed that the flocculation phenotype induced byCAD1/AAF1 was similar to Flo1. Thus,CAD1/AAF1 probably encodes a regulatory protein that stimulates endothelial cell adherence inS. cerevisiae by inducing a flocculation phenotype. WhetherCAD1/AAF1 contributes to the adherence ofC. albicans to endothelial cells remains to be determined.

infection and immunity

Cloning and Characterization ofCAD1/AAF1, a Gene fromCandida albicansThat Induces Adherence to Endothelial Cells after Expression inSaccharomyces cerevisiae