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Several immunodominant major proteins ranging from 23 to 30 kDa were identified in the outer membrane fractions ofEhrlichia chaffeensis andEhrlichia canis . The N-terminal amino acid sequence of a 28-kDa protein ofE. chaffeensis (one of the major proteins) was determined. The gene (p28 ), almost full length, encoding the 28-kDa protein was cloned by PCR with primers designed based on the N-terminal sequence of theE. chaffeensis 28-kDa protein and the consensus sequence between the C termini of theCowdria ruminantium MAP-1 andAnaplasma marginale MSP-4 proteins. Thep28 gene was overexpressed, and antibody to the recombinant protein was raised in a rabbit. The antibody and serum from a patient infected withE. chaffeensis reacted with the recombinant protein, three proteins (29, 28, and 25 kDa) ofE. chaffeensis , and a 30-kDa protein ofE. canis . Immunoelectron microscopy with the rabbit antibody revealed that the antigenic epitope of the 28-kDa protein was exposed on the surface ofE. chaffeensis . Southern blot analysis with a32 P-labeledp28 gene probe revealed multiple copies of genes homologous top28 in theE. chaffeensis genome. Six copies of thep28 gene were cloned and sequenced from the genomic DNA by using the same probe. The open reading frames of these gene copies were tandemly arranged with intergenic spaces. They were nonidentical genes and contained a semivariable region and three hypervariable regions in the predicted protein molecules. One of the gene copies encoded a protein with an internal amino acid sequence identical to the chemically determined N-terminal amino acid sequence of a 23-kDa protein ofE. chaffeensis . Immunization with the recombinant P28 protein protected mice from infection withE. chaffeensis . These findings suggest that the 30-kDa-range proteins ofE. chaffeensis represent a family of antigenically related homologous proteins encoded by a single gene family.

Immunodominant Major Outer Membrane Proteins of Ehrlichia chaffeensis Are Encoded by a Polymorphic Multigene Family