English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Adherence of enteropathogenicEscherichia coli (EPEC) to epithelial cells is dependent on a type IV fimbria, termed the bundle-forming pilus (BFP). A cluster of 14 genes is required for expression of BFP. The eighth gene in the cluster,bfpF , encodes a putative nucleotide-binding protein which resembles the PilT protein ofPseudomonas aeruginosa . It has been proposed that PilT is required for the retraction of theP. aeruginosa pilus, which results in twitching motility. To test the role of BfpF in BFP function and EPEC pathogenesis, two different mutations were constructed in thebfpF gene, one in the cloned gene cluster in a laboratoryE. coli strain and one in wild-type EPEC. Neither mutation affected prepilin synthesis, leader sequence processing, or pilus biogenesis. However, both mutations resulted in increased localized adherence. In addition, the EPECbfpF mutant displayed increased aggregation. The EPECbfpF mutant was not deficient in attaching and effacing activity or invasion capacity. These results suggest that BfpF decreases aggregation and adherence by EPEC but that subsequent steps in EPEC pathogenesis do not require this protein.

infection and immunity

Role of BfpF, a Member of the PilT Family of Putative Nucleotide-Binding Proteins, in Type IV Pilus Biogenesis and in Interactions between Enteropathogenic <i>Escherichia coli</i> and Host Cells

Role of BfpF, a Member of the PilT Family of Putative Nucleotide-Binding Proteins, in Type IV Pilus Biogenesis and in Interactions between Enteropathogenic Escherichia coli and Host Cells