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A simple two-step procedure is described for the concentration and purification of hepatitis B antigen. The antigen is precipitated by polyethylene glycol and then adsorbed on and eluted from an insoluble polyelectrolyte, polyelectrolyte 60, which is a cross-linked copolymer of isobutylene maleic anhydride.

infection and immunity

Concentration and Purification of Hepatitis B Antigen with Polyethylene Glycol and Polyelectrolyte 60, a Cross-Linked Copolymer of Isobutylene Maleic Anhydride