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Increased anti-core glycolipid antibody binding was visualized by immunoelectron microscopy after incubation of Escherichia coli K1:O7 cells with a bacteriolytic antibiotic and compared with binding in control cells. The findings suggest that the core glycolipid regions of the lipopolysaccharides of some gram-negative bacilli can be effectively sequestered until liberated by antibiotic-induced cell lysis.

Immunolabeling of lipopolysaccharide liberated from antibiotic-treated Escherichia coli