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A mouse model of influenza infection was studied to help define parameters that may affect serodiagnosis of human infections by immunoassays. Antibodies to both type- and subtype-specific influenza A antigens were measured by a solid-phase immunofluorometric assay. Dilute mouse sera were added to purified influenza virus that had been covalently bound to polyaminostyrene microbeads, and the bound antibody was detected by fluorescein isothiocyanate-labeled isotype-specific antisera. Results were consistent in that upon exposure of mice by either infection alone or by vaccination after infection, both immunoglobulin M (IgM) and IgG antibodies reactive with newly encountered subtype specific viral antigens were measured. IgG antibody was usually detectable by the solid-phase immunofluorometric assay several days before it could be detected by a hemagglutination inhibition test. Increased levels of antibody of the IgG1, IgGa, IgG2b, and IgG3 subclasses were also measured during influenza infection. Surprisingly, response to type-specific viral antigens was of the IgG class in primary as well as in secondary exposure. The results suggest that for serodiagnosis of influenza infections by detection of specific IgM antibody, the assay should use subtype-specific antigens.

Immunoglobulin M and G antibody response to type- and subtype-specific antigens after primary and secondary exposures of mice to influenza A viruses