Open AccessSecretion of Functional Monocyte Chemotactic Protein 3 by RecombinantMycobacterium bovisBCG Attenuates Vaccine Virulence and Maintains Protective Efficacy againstM. tuberculosisInfection
Author(s) -
Anthony A. Ryan,
Joanne M. Spratt,
Warwick J. Britton,
James A. Triccas
Publication year - 2006
Publication title -
infection and immunity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.508
H-Index - 220
eISSN - 1070-6313
pISSN - 0019-9567
DOI - 10.1128/iai.00897-06
Subject(s) - mycobacterium bovis , biology , virulence , vaccination , microbiology and biotechnology , secretion , mycobacterium tuberculosis , monocyte , immunology , bcg vaccine , antigen , tuberculosis , in vivo , virology , recombinant dna , medicine , pathology , gene , biochemistry
A strain of Mycobacterium bovis BCG that secretes high levels of functional murine monocyte chemotactic protein 3 (BCG(MCP-3)) was developed. Mice vaccinated with BCG(MCP-3) displayed increased lymphocyte migration in vivo and augmented antigen-specific T-cell responses compared to mice vaccinated with BCG alone. The level of protection afforded by BCG(MCP-3) was equivalent to that with control BCG; however, immunodeficient mice infected with BCG(MCP-3) survived significantly longer than mice infected with the control BCG strain. Therefore, BCG(MCP-3) may be a safer alternative than conventional BCG for vaccination of immunocompromised individuals.
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