English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Triosephosphate isomerase ofTrichomonas vaginalis (Tv TIM) is a 27-kDa cytoplasmic protein encoded by two genes,tvtim1 andtvtim2 , that participates in glucose metabolism.Tv TIM is also localized to the parasite surface. Thus, the goal of this study was to identify the novel functions of the surface-associatedTv TIM inT. vaginalis and to assess the effect of glucose as an environmental factor that regulates its expression and localization. Reverse transcription-PCR (RT-PCR) showed that thetvtim genes were differentially expressed in response to glucose concentration.tvtim1 was overexpressed under glucose-restricted (GR) conditions, whereastvtim2 was overexpressed under glucose-rich, or high-glucose (HG), conditions. Western blot and indirect immunofluorescence assays also showed that glucose positively affected the amount and surface localization ofTv TIM inT. vaginalis . Affinity ligand assays demonstrated that the recombinantTv TIM1 andTv TIM2 proteins bound to laminin (Lm) and fibronectin (Fn) but not to plasminogen. Moreover, higher levels of adherence to Lm and Fn were detected in parasites grown under HG conditions than in those grown under GR conditions. Furthermore, pretreatment of trichomonads with an anti-Tv TIMr polyclonal antibody or pretreatment of Lm- or Fn-coated wells with both recombinant proteins (Tv TIM1r andTv TIM2r) specifically reduced the binding of live parasites to Lm and Fn in a concentration-dependent manner. Moreover,T. vaginalis was exposed to different glucose concentrations during vaginal infection of women with trichomoniasis. Our data indicate thatTv TIM is a surface-associated protein under HG conditions that mediates specific binding to Lm and Fn as a novel virulence factor ofT. vaginalis .

infection and immunity

The Glycolytic Enzyme Triosephosphate Isomerase of Trichomonas vaginalis Is a Surface-Associated Protein Induced by Glucose That Functions as a Laminin- and Fibronectin-Binding Protein