Open AccessComplete Draft Genome Sequence of Escherichia coli KRX, a Strain for Efficient Cloning and High-Yield Expression of Proteins under Control of the T7 RNA Polymerase
Author(s) -
Jan-Philipp Schwarzhans,
Daniel Wibberg,
Anika Winkler,
Jörn Kalinowski,
Karl Friehs
Publication year - 2017
Publication title -
genome announcements
Language(s) - English
Resource type - Journals
ISSN - 2169-8287
DOI - 10.1128/genomea.00933-17
Subject(s) - cloning (programming) , escherichia coli , biology , strain (injury) , t7 rna polymerase , rna polymerase , genome , transformation (genetics) , computational biology , whole genome sequencing , genetics , gene , computer science , bacteriophage , anatomy , programming language
Escherichia coli KRX is a strain offering both a high transformation efficiency and the possibility to produce the target protein to high yields in one host, avoiding additional cloning steps. Here, the draft genome sequence of E. coli KRX is presented and provides the genetic basis for additional biotechnological applications.
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