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A chimeric yellow fever virus/West Nile virus (WNV) was compared to WNV alone as a biosafety level 2 reagent in the plaque reduction neutralization test for determining WNV infection histories. Concordance was 96.3% among 188 avian and equine serum samples. Neutralizing antibody titers were frequently more than twofold lower with the chimera.

Use of a Surrogate Chimeric Virus To Detect West Nile Virus-Neutralizing Antibodies in Avian and Equine Sera