Open AccessComparison of Enzyme Immunoassays for Detection of Antibodies to Hepatitis D Virus in Serum
Author(s) -
Siu-Kei Chow,
Ederlyn E. Atienza,
Linda Cook,
Harry E. Prince,
Patricia Slev,
Mary Lapé-Nixon,
Keith R. Jerome
Publication year - 2016
Publication title -
clinical and vaccine immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.649
H-Index - 77
eISSN - 1556-6811
pISSN - 1556-679X
DOI - 10.1128/cvi.00028-16
Subject(s) - serology , antibody , virology , hepatitis a virus , immunoassay , medicine , virus , immunology
Serology remains critical for diagnosing hepatitis D virus (HDV) infection, which affects 15 to 20 million people worldwide, but the literature on characterizing commercial enzyme immunoassays (EIAs) dates back to 15 years ago. We evaluated 2 commercial EIAs currently available for detecting anti-HDV antibodies. The DiaSorin assay demonstrated 100% sensitivity and specificity. Using a modified cutoff value, the Cusabio assay demonstrated a sensitivity of 81.3% and specificity of 90.9%. Our data show that recently developed EIAs are reliable for anti-HDV antibody detection.
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