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We developed a polysaccharide-specific flow cytometric opsonophagocytic assay (OPA) for the simultaneous measurement of functional antibody to Neisseria meningitidis serogroups A, C, Y, and W135. OPA titers significantly correlated with serum bactericidal assay titers for all serogroups tested (mean r = 0.96; P &lt; 0.001). OPA could be used in meningococcal vaccine evaluation.

clinical and vaccine immunology

Opsonophagocytosis of Fluorescent Polystyrene Beads Coupled to <i>Neisseria meningitidis</i> Serogroup A, C, Y, or W135 Polysaccharide Correlates with Serum Bactericidal Activity

Opsonophagocytosis of Fluorescent Polystyrene Beads Coupled to Neisseria meningitidis Serogroup A, C, Y, or W135 Polysaccharide Correlates with Serum Bactericidal Activity