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Four of 48 raw milk samples contained catalase-negative, gram-positive, motile, sporeforming, rod-shaped bacteria that grew optimally at 22 to 30 C and slowly at low temperatures. Isolates from two samples had a minimal growth temperature of 4 C, were anaerobic, and had characteristics similar toClostridium hastiforme ; those from the other two samples had a minimal growth temperature of 0  1 C, were anaerobic, aerotolerant, and had characteristics similar toC. carnis . Specific growth rates, doubling times, ability to grow in pasteurized milk stored in commercial cartons, and resistance of spores to heating were determined for one strain ofC. hastiforme .

Isolation and Identification of Psychrophilic Species of Clostridium from Milk