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In a study of the effect of glycerin in transport media onVibrio parahaemolyticus andSalmonella , it was found that a concentration of 30% glycerin was highly inhibitory forV. parahaemolyticus and to a lesser degree forSalmonella . The incorporation of peptone or human feces in media did not reduce the inhibitory effect of glycerin. In media with 15% glycerin, viable counts ofV. parahaemolyticus andSalmonella increased after 24 hr of incubation both in the presence and absence of feces. Due to the concurrent increase in the total bacterial count in the media containing feces, no enrichment effect was noted.

Inhibitory Effect of Glycerin on Vibrio parahaemolyticus and Salmonella