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A microbiological assay was developed for bleomycin, an antitumor antibiotic reported to be active in human trials. The assay bacterium was a strain ofEscherichia coli which is resistant to ethionine. Studies revealed relatively high concentrations of bleomycin in the blood and urine of mice after a single dose, &lt; 0.33ld 10 , injected intraperitoneally.

Bleomycin, an Antitumor Antibiotic: Improved Microbiological Assay and Tissue Distribution Studies in Normal Mice