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Acid-resistant, nonenveloped viruses belonging to the enterovirus, reovirus, and adenovirus groups were readily concentrated on PE60, an insoluble cross-linked polyelectrolyte based on isobutylene maleic anhydride. Hydrolysis of PE60 by NaOH increased its capacity to adsorb viruses. Hydrogen ion levels played an important role in virus concentration; optimalp H levels for maximal virus adsorption were betweenp H 3.0 and 4.5. Undiluted virus was easily concentrated from large volumes on PE60, and the adsorbed virus was readily eluted atp H 8 to 9.

Concentration and Purification of Viruses by Adsorption to and Elution from Insoluble Polyelectrolytes