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Seventy-one clinical isolates of 5 species of group DStreptococcus (Enterococcus) were tested for susceptibility to 15 antibiotics atp H 5.0, 7.4, and 8.5. Penicillin G, ampicillin, cephalothin, cephaloridine, and novobiocin were considerably more active against all strains atp H 5.0 than in the more alkaline media. On the other hand, lincomycin, clindimycin, erythromycin, and gentamicin were moderately to markedly more active atp H 8.5. No important differences were noted in the susceptibility of the strains to kanamycin and streptomycin, at thep H levels tested, but the organisms were quite resistant to them in these tests. The various species differed quantitatively in their susceptibility to the individual drugs and in the effects ofp H. The size of the inoculum also had a variable effect on susceptibility, depending on the species ofEnterococcus , the antibiotics, and thep H of the test medium. The data suggest that, in the antibiotic treatment of urinary tract infections caused byEnterococcus , attempts should be made to achieve the optimump H in the urine, particularly in view of the fact that organisms of this group are often resistant to several antibiotics in the usual tests.

Effect of p H of Medium and Size of Inoculum on Activity of Antibiotics Against Group D Streptococcus (Enterococcus)