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The passage ofTreponema pallidum through commercially available Millipore membrane filters of various pore sizes was examined. No microscopically detectable organisms passed through a filter with a pore diameter of 0.22 μm. As pore size was increased, progressively more organisms passed through. Motile organisms passed through filters to a greater extent than nonmotile ones; however, 22% of the motile and 50% of the nonmotileT. pallidum organisms did not pass through the largest pore diameter tested (14.0 μm). Filtration ofT. pallidum suspensions through membrane filters may offer a way of separating the organisms from larger particles of debris which accompany their extraction from rabbit testicular syphilomas.

Passage of Treponema pallidum Through Membrane Filters of Various Pore Sizes