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Single-nucleotide polymorphisms (SNPs) are targets to discriminate intraspecies diversity of bacteria and to correlate a genotype with a potential pathotype. Quantification of polygenotypic populations supports this task for in vitro and in vivo applications. We present a novel assay capable of quantifying mixtures of two genotypes differing by only one SNP.

applied and environmental microbiology (print)

Single-Nucleotide-Polymorphism-Specific PCR for Quantification and Discrimination of <i>Chlamydia pneumoniae</i> Genotypes by Use of a “Locked” Nucleic Acid

Single-Nucleotide-Polymorphism-Specific PCR for Quantification and Discrimination of Chlamydia pneumoniae Genotypes by Use of a “Locked” Nucleic Acid