Both msa Genes in Renibacterium salmoninarum Are Needed for Full Virulence in Bacterial Kidney Disease | Zendy

Alison Coady | Zendy; Anthony L. Murray | Zendy; Diane G. Elliott | Zendy; Linda D. Rhodes | Zendy

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Both msa Genes in Renibacterium salmoninarum Are Needed for Full Virulence in Bacterial Kidney Disease

Author(s) -

Alison Coady,

Anthony L. Murray,

Diane G. Elliott,

Linda D. Rhodes

Publication year - 2006

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.72.4.2672-2678.2006

Subject(s) - virulence , biology , microbiology and biotechnology , gene , mutagenesis , gene duplication , oncorhynchus , mutant , plasmid , virulence factor , gene expression , genetics , fish <actinopterygii> , fishery

Renibacterium salmoninarum , a gram-positive diplococcobacillus that causes bacterial kidney disease among salmon and trout, has two chromosomal loci encoding the major soluble antigen (msa ) gene. Because the MSA protein is widely suspected to be an important virulence factor, we used insertion-duplication mutagenesis to generate disruptions of either themsa1 ormsa2 gene. Surprisingly, expression of MSA protein in broth cultures appeared unaffected. However, the virulence of either mutant in juvenile chinook salmon (Oncorhynchus tshawytscha ) by intraperitoneal challenge was severely attenuated, suggesting that disruption of themsa1 ormsa2 gene affected in vivo expression.

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