Open AccessOverexpression of the 14α-Demethylase Target Gene ( CYP51 ) Mediates Fungicide Resistance in Blumeriella jaapii
Author(s) -
Zhonghua Ma,
Tyre J. Proffer,
Janette L. Jacobs,
George W. Sundin
Publication year - 2006
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.72.4.2581-2585.2006
Subject(s) - efflux , biology , fungicide , demethylase , gene , demethylation , gene expression , retrotransposon , pathogen , sterol , genetics , microbiology and biotechnology , transposable element , botany , biochemistry , cholesterol , mutant , dna methylation , epigenetics
Sterol demethylation inhibitor (DMI) fungicides are widely used to control fungi pathogenic to humans and plants. Resistance to DMIs is mediated either through alterations in the structure of the target enzyme CYP51 (encoding 14α-demethylase), through increased expression of theCYP51 gene, or through increased expression of efflux pumps. We found thatCYP51 expression in DMI-resistant (DMIR ) isolates of the cherry leaf spot pathogenBlumeriella jaapii was increased 5- to 12-fold compared to that in DMI-sensitive (DMIS ) isolates. Analysis of sequences upstream ofCYP51 in 59 DMIR isolates revealed that various forms of a truncated non-long terminal direct repeat long interspersed nuclear element retrotransposon were present in all instances. Similar inserts upstream ofCYP51 were not present in any of 22 DMIS isolates examined.
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