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Growth of a glutamine synthetase-deficient mutant of Streptococcus thermophilus was compared to that of the parent strain in milk that was not supplemented or was supplemented with ammonium chloride, glutamine, or the urease inhibitor flurofamide. It was concluded that one of the functions of urease is to supply ammonia for the synthesis of glutamine.

Glutamine Synthesis Is Essential for Growth of Streptococcus thermophilus in Milk and Is Linked to Urea Catabolism