Open AccessPurification and cDNA Cloning of NADPH-Dependent Aldoketoreductase, Involved in Asymmetric Reduction of Methyl 4-Bromo-3-Oxobutyrate, from Penicillium citrinum IFO4631
Author(s) -
Hiroyuki Asako,
Ryuhei Wakita,
Kenji Matsumura,
Masatoshi Shimizu,
Jun Sakai,
Nobuya Itoh
Publication year - 2005
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.71.2.1101-1104.2005
Subject(s) - penicillium citrinum , complementary dna , escherichia coli , reductase , clone (java method) , biology , microbiology and biotechnology , strain (injury) , chemistry , biochemistry , enzyme , dna , gene , botany , anatomy
Penicillium citrinum was found to catalyze the reduction of methyl 4-bromo-3-oxobutyrate to methyl (S)-4-bromo-3-hydroxybutyrate [(S)-BHBM] with high optical purity. From the strain, a cDNA clone encoding a novel NADPH-dependent alkyl 4-halo-3-oxobutyrate reductase (KER) was isolated. Escherichia coli cells overexpressing KER produced (S)-BHBM in the presence of an NADPH-regeneration system.
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