Open AccessInfection of Mice with Aerosolized Mycobacterium tuberculosis : Use of a Nose-Only Apparatus for Delivery of Low Doses of Inocula and Design of an Ultrasafe Facility
Author(s) -
J. Reid Schwebach,
Bing Chen,
Aharona GlatmanFreedman,
Arturo Casadevall,
John D. McKinney,
John L. Harb,
Patrick J. McGuire,
William Barkley,
Barry R. Bloom,
William R. Jacobs
Publication year - 2002
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.68.9.4646-4649.2002
Subject(s) - aerosolization , mycobacterium tuberculosis , tuberculosis , nose , medicine , microbiology and biotechnology , virology , biology , surgery , pathology , inhalation , anesthesia
Aerosolized delivery of virulent or hypervirulent Mycobacterium tuberculosis requires careful consideration of methodology and safety. To maximize safety, we installed a nose-only aerosol apparatus that can reproducibly deliver a low dose (<100 CFU per mouse) of M. tuberculosis in a carefully designed biohazard facility.