Open AccessPCR-Restriction Fragment Length Polymorphism Analysis of a Diagnostic 452-Base-Pair DNA Fragment Discriminates between Cryptosporidium parvum and C. meleagridis and between C. parvum Isolates of Human and Animal Origin
Author(s) -
Karine Guyot,
A. Follet-Dumoulin,
C. Recourt,
Etienne Lelièvre,
J. C. Cailliez,
Eduardo DeiCas
Publication year - 2002
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.68.4.2071-2076.2002
Subject(s) - cryptosporidium parvum , biology , genotype , restriction fragment length polymorphism , locus (genetics) , genomic dna , cryptosporidium , restriction fragment , genetics , microbiology and biotechnology , restriction enzyme , ribosomal rna , ribosomal dna , polymerase chain reaction , dna , gene , phylogenetics , feces
Genomic DNAs from human Cryptosporidium isolates previously typed by analysis of the 18S ribosomal DNA locus (Cryptosporidium parvum bovine genotype, C. parvum human genotype, Cryptosporidium meleagridis, and Cryptosporidium felis) were used to amplify the diagnostic fragment described by Laxer et al. (M. A. Laxer, B. K. Timblin, and R. J. Patel, Am. J. Trop. Med. Hyg., 45:688-694, 1991). The obtained 452-bp amplified fragments were sequenced and aligned with the homologous Cryptosporidium wrairi sequence. Polymorphism was exploited to develop a restriction fragment length polymorphism method able to discriminate Cryptosporidium species and C. parvum genotypes.
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