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TherulAB locus confers tolerance to UV radiation and is borne on plasmids of the pPT23A family inPseudomonas syringae. We sequenced 14rulA alleles fromP. syringae strains representing seven pathovars and found sequence differences of 1 to 12% within pathovar syringae, and up to 15% differences between pathovars. Since the sequence variation withinrulA was similar to that ofP. syringae chromosomal alleles, we hypothesized thatrulAB has evolved over a long time period inP. syringae. A phylogenetic analysis of the deduced amino acid sequences ofrulA resulted in seven clusters. Strains from the same plant host grouped together in three cases; however, strains from different pathovars grouped together in two cases. In particular, therulA alleles fromP. syringae pv. lachrymans andP. syringae pv. pisi were grouped but were clearly distinct from the other sequenced alleles, suggesting the possibility of a recent interpathovar transfer. We constructed chimericrulAB expression clones and found that the observed sequence differences resulted in significant differences in UV (wavelength) radiation sensitivity. Our results suggest that specific amino acid changes in RulA could alter UV radiation tolerance and the competitiveness of theP. syringae host in the phyllosphere.

applied and environmental microbiology

Sequence Diversity of rulA among Natural Isolates of Pseudomonas syringae and Effect on Function of rulAB -Mediated UV Radiation Tolerance