Open AccessSpecific Detection of Leuconostoc mesenteroides subsp. mesenteroides with DNA Primers Identified by Randomly Amplified Polymorphic DNA Analysis
Author(s) -
Giancarlo Moschetti,
Giuseppe Blaiotta,
Francesco Villani,
Salvatore Coppola
Publication year - 2000
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.66.1.422-424.2000
Subject(s) - leuconostoc mesenteroides , primer (cosmetics) , biology , dna , leuconostoc , genetics , microbiology and biotechnology , strain (injury) , bacteria , chemistry , lactic acid , organic chemistry , anatomy
Randomly amplified polymorphic DNA analysis using primer 239 (5′ CTGAAGCGGA 3′) was performed to characterizeLeuconostoc sp. strains. All the strains ofLeuconostoc mesenteroides subsp.mesenteroides (with the exception of two strains), two strains formerly identified asL. gelidum , and one strain ofLeuconostoc showed a common band at about 1.1 kb. This DNA fragment was cloned and sequenced in order to verify its suitability for identifyingL. mesenteroides subsp.mesenteroides strains.