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TheTri5 gene encodes trichodiene synthase, which catalyzes the first reaction in the trichothecene biosynthetic pathway. In vitro, a direct relationship was observed betweenTri5 expression and the increase in deoxynivalenol production over time. We developed a reverse transcription (RT)-PCR assay to quantifyTri5 gene expression in trichothecene-producing strains ofFusarium species. We observed an increase inTri5 expression following treatment ofFusarium culmorum with fungicides, and we also observed an inverse relationship betweenTri5 expression and biomass, as measured by β-d -glucuronidase activity, during colonization of wheat (cv. Avalon) seedlings byF. culmorum . RT-PCR analysis also showed that for ears of wheat cv. Avalon inoculated withF. culmorum , there were different levels ofTri5 expression in grain and chaff at later growth stages. We used theTri5 -specific primers to develop a PCR assay to detect trichothecene-producingFusarium species in infected plant material.

Development and Use of a Reverse Transcription-PCR Assay To Study Expression of Tri5 by Fusarium Species In Vitro and In Planta