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Pseudomonas aeruginosa accumulates polyphosphates in response to nutrient limitations. To elucidate the function of polyphosphate in this microorganism, we have investigated polyphosphate metabolism by isolating fromP. aeruginosa 8830 the genes encoding polyphosphate kinase (PPK) and exopolyphosphatase (PPX), which are involved in polyphosphate synthesis and degradation, respectively. The 690- and 506-amino-acid polypeptides encoded by the two genes have been expressed inEscherichia coli and purified, and their activities have been tested in vitro. Gene replacement was used to construct a PPK-negative strain ofP. aeruginosa 8830. Low residual PPK activity in theppk mutant suggests a possible alternative pathway of polyphosphate synthesis in this microorganism. Primer extension analysis indicated thatppk is transcribed from a ςE -dependent promoter, which could be responsive to environmental stresses. However, no coregulation betweenppk andppx promoters has been demonstrated in response to osmotic shock or oxidative stress.

Cloning and Characterization of Polyphosphate Kinase and Exopolyphosphatase Genes from Pseudomonas aeruginosa 8830