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Pseudomonas putida grown in continuous culture on benzoate or m-toluate lost the ability to grow on benzoate or m-toluate plates. A similar effect was not seen with a glucose continuous culture. Cells carrying and expressing a TOL plasmid rapidly lost their ability to grow on benzoate solid medium.

Impact of Growth in Benzoate and m-Toluate Liquid Media on Culturability of Pseudomonas putida on Benzoate and m-Toluate Plates